ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
PRODUCTION OF HAPLOID AND DOUBLED HAPLOID PLANTS FROM ANTHER-DERIVED CALLUS OF LILIUM FORMOSANUM |
| Authors: | D.-S. Han, Y. Niimi |
| Keywords: | anther culture, haploid callus, lily, plant regeneration |
| Abstract:
Anther cultures of Lilium formosanum were made to obtain haploid and doubled haploid plants. Anthers containing microspores at the uninucleate stage were cultured on MS medium supplemented with picloram and zeatin. Five haploid callus lines were obtained and maintained long term by subculturing them at 2- to 3-month intervals on MS medium containing picloram. Haploid plants (2n = x = 12) were regenerated from the anther-derived callus lines and grown to flowering. These haploid plants showed poor growth and abnormal meiosis resulting in formation of sterile pollen. Doubled haploid plants were produced from a colchicine-treated haploid callus line, but pollen of the doubled haploid plants also was sterile. The ploidy level of the callus lines was determined by flow cytometry. Five anther-derived callus lines which had been maintained over 4 years showed a difference in ploidy: 2 lines remained at a haploid level, 2 lines had became diploid and one line was a mixoploid population consisting of haploid, diploid and triploid cells. | |
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