ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
TECHNIQUES FOR THE IN VITRO PROPAGATION OF RHODOPHIALA AND LEUCOCORYNE SPP. |
| Authors: | E. Olate, M. Bridgen |
| Keywords: | geophyte, floriculture, plant tissue culture, propagation |
| Abstract:
Leucocoryne coquimbensis and Rhodophiala bagnoldii are endogenous geophytes from Chile that have the potential to be used as cut flower crops, potted plants, and garden plants. However, traditional propagation techniques with these species can be difficult. Different mechanical, environmental, and chemical treatments were used in vitro to determine the optimum method to increase bulb production rates. Both bulbs and seeds of each species were used. Traditional propagation techniques that are used to increase the number of bulbs were attempted under in vitro conditions with the Leucocoryne and Rhodophiala. These include scooping, scoring, and sectioning of the basal plate. Scoring and sectioning techniques increased the number of bulblets produced in vitro, but scooping had no advantage. With Rhodophiala, the effects of the cytokinins BA (N6-benzyladenine) and 2iP (2-isopentenyl-adenine) each at 2.5, 5.0, and 10.0 μM were tested. BA was shown to be advantageous in vitro, but there was no advantage to incorporating 2iP in the propagation medium. The effect of sucrose level in the medium on the growth of Rhodophiala bulbs was tested in vitro. The concentrations 30, 60, and 90 grams per liter were evaluated as well as a control with no sucrose. There is increased bulb growth of Rhodophiala in vitro when increased levels of sucrose are added to the medium. However, the addition of higher levels of sucrose does not affect the number of bulbs that are produced. This research determined that it is possible to propagate Leucocoryne and Rhodophiala bulbs in vitro. | |
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