ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
THE BREEDING IN POLYKARYOMORPHIC PROGENIES OF SPIDER LILY (LYCORIS SPP.) MEDIATED SYNTHETIC DIKARYOTYPE HYBRIDS 2n+18 (4M+3T+11A) |
| Authors: | M.C. Wu, M.S. Yuan, C.T. Shii |
| Abstract:
The interspecific hybridization between native golden spider lily (Lycoris aurea) 2n = 14 (8M+6T) and four monomorphic A karyotype species 2n = 22 (22A) achieved the same trimorphic M-T-A hybrids 2n = 18 (4M+3T+11A). All these synthetic dikaryotype hybrids exhibit female infertility, but, partial male fertility, capable to reproduce 24.7-29.1% of functional male gametes. The paternal dikaryotype hybrids were test crossed with M-T and A karyotype species. 172 test progenies were rescued by embryo culture, 94.2% of recovered progenies were cytologically examined as 2n = 14, 15, 16, 17, 18, 19, 20, 21 and 22. Each of the same numerical classes was also distinguishable into several karyomorphs, and constituted of constant 22 total large arms, considered as genetic balance eudiploid. 16% of diploid plants contain altered chromosomes such as arm elongation or shortening, supra T, extra mini-metacentrics or fragment. The other 3.49% recovered progenies were deviated from constant 22 arms categorized as aneuploid. Additionally, four M-T-A triploid plants (2.33%) of 25 = 8M+6T+11A, 26 = 7M+8T+11A, 27 = 6M+4T+17A+1m and 30 = 5M+3T+22A were also achieved. It was determined that the synthetic dikaryotype hybrids were dynamic rather than fixed heterozygosity. It was possible to create numerous neokaryotypes and thus accelerate genetic diversification, which would benefit breeding selection of desirable elite cultivars. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
