ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
NEW ASPECTS OF TULIP BREEDING: EMBRYO CULTURE AND POLYPLOID |
| Author: | K. Okazaki |
| Keywords: | bulblet formation, chromosome doubling, interspecific hybridization, in vitro, meiosis, nitrous oxide, 2n pollen |
| Abstract:
In order to improve bulb productivity and ornamental characteristics in tulips, it is necessary to increase genetic variation by using breeding techniques such as overcoming of interspecific barrier, embryo rescue of abortive embryos and induction of polyploids. To identify interspecific barrier between species, crossing experiments have been studied about fifty years ago and recently embryo rescue technique just has been attempted in Holland. In polyploid breeding, colchicine treatment is a common technique to produce polyploids, whereas nitrous oxide has been applied to zygotes as a polyploidizing agent in various crops. This paper reviews some of the literature and our results regarding to embryo rescue technique and also reports on the usefulness of 2n pollen induced with nitrous gas in tulip breeding. | |
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