ISHS Acta Horticulturae 671: IX International Pear Symposium
DEVELOPMENT OF MOLECULAR MARKERS LINKED TO SEVERAL FRUIT TRAITS IN ORIENTAL PEAR |
| Authors: | D. Kim, J.H. Hwang, Y.U. Shin, I.S. Shin, H.J. Lee, S.S. Hong, S.J. Kang |
| Keywords: | Pyrus pyrifolia, P. ussuriensis, BSA, molecular marker, RAPD, AFLP |
| Abstract:
To develop molecular markers linked to fruit traits, several important fruit characteristics such as skin color, the amount of grit cells, hardness, core size and weight were assessed and genetically analyzed in 135 F1 pear seedlings from the cross between ‘Niitaka’ (P. pyrifolia Nakai) and ‘Suhyangri’ (P. ussuriensis Maxim.). All the characteristics investigated in this study, except the grit cell content, showed a normal distribution. To detect molecular markers linked to several fruit characteristics, high quality genomic DNAs were extracted and sorted into two DNA pools by bulked segregant analysis. We selected two random amplified polymorphic DNA (RAPD) and seven amplified fragment length polymorphism (AFLP) markers. OPD05-850 and OPD08-500, linked to fruit hardness and skin color respectively, were selected by RAPD analysis. Eight AFLP markers out of the 64 primer combinations were selected. Those were EaaMcag-440 and EaggMcag-470 linked to skin color, EacaMcac-259, EacaMctt-330, and EagcMcta-170 linked to grit cell content, EaacMctg-350 linked to fruit hardness, EacaMctt-340 and EaagMctg-330 linked to fruit shape. The selected markers were cloned and sequenced to for use as stable co-dominant markers for the increase of pear breeding efficiency. The two AFLP markers linked with the amount of grit cells were sequenced successfully from EacaMcac-259 and EacaMctt-330 with their original marker sequences. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
