ISHS Acta Horticulturae 67: X International Symposium on Fruit Tree Virus Diseases
THE DETECTION OF PLUM POX AND OTHER VIRUSES IN WOODY PLANTS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) |
| Authors: | M.F. Clark, A.N. Adams, J.M. Thresh, R. Casper |
| Abstract:
The new technique of enzyme-linked immunosorbent assay (ELISA) was used successfully to detect plum pox virus in the fruits, flowers, leaves and roots of plum. Numerous samples from commercial nurseries and orchards and from experimental plants in the field and glasshouse were tested quickly and conveniently. The sensitivity of the technique and its suitability for handling many small samples of tissue were exploited in assessing differences in virus content within leaves and between different floral parts. Arabis mosaic virus was detected by ELISA in extracts made direct from blackcurrant leaves and buds and from the leaves of raspberry, strawberry, elderberry and ivy. It was also possible to detect apple mosaic virus in apple flowers and in leaves collected in spring and early summer when leaf extracts failed to infect cucumber test plants that were inoculated mechanically. The success of ELISA for detecting viruses in woody hosts is discussed in relation to other possible applications of the technique. | |
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