ISHS Acta Horticulturae 668: I International Humulus Symposium
CONSTRUCTION OF THE GENETIC LINKAGE MAP AND QTL ANALYSIS ON HOP (HUMULUS LUPULUS L.) |
| Authors: | K. Koie, A. Inaba, Y. Okada, T. Kaneko, K. Ito |
| Keywords: | AFLP, pseudo-test cross, out bred, MAS, alpha acid, beta acid, xanthohumol, essential oil |
| Abstract:
Marker assisted selection (MAS), could improve the breeding of hop (Humulus lupulus L.) We would be able to select for mature plant traits in seedlings, to evaluate the genetic potential of male plants, and to make selections without the effects of environmental factors. In this preliminary study for the application of MAS to hop breeding, genetic linkage maps were constructed using amplified fragment length polymorphisms (AFLP), and quantitative trait loci (QTL) for the contents of hop cone chemical components. The hop cultivar Chinook, the male parent ‘SaM’ and their female progenies (n=130) were used in this experiment. The genetic linkage maps of Chinook and SaM were constructed with 135 and 33 markers, respectively. The total map length was 272 cM consisting of 10 linkage groups and 102 cM of 7 linkage groups for Chinook and SaM, respectively. The interval mapping of QTL was carried out with chromatographic quantifications of nine chemical components from the crops in 1999, 2000 and 2002. Twelve QTLs were detected for 7 chemical components such as alpha acids, beta acids, xanthohumol, cohumulone ratio to alpha acids, colupulone ratio to beta acids,  -caryophyllene and  -farnesene. All QTLs in this study are not detected independently but are overlapped with those of the other chemical components in 4 linkage regions.
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