ISHS Acta Horticulturae 668: I International Humulus Symposium
GENBANK-DERIVED MICROSATELLITE MARKERS IN HOP |
| Authors: | N.V. Bassil, B. Gilmore, J. Oliphant, K. Hummer, J.A. Henning |
| Keywords: | Humulus lupulus L., fingerprint, simple sequence repeats (SSR) |
| Abstract:
A method was developed to identify microsatellite-containing sequences from existing Humulus lupulus L. entries in the Nucleotide GenBank database. We screened 45 genomic, mRNA and expressed sequence tag (EST) Humulus nuclear sequences and identified 7 that contained simple sequence repeats (SSRs). Primer pairs were designed for 15 sequences and the optimum annealing temperature was determined by gradient PCR. SSR primers were screened for polymorphism in 24 wild accessions of hops. Ten primer pairs generated repeatable polymorphic bands and seemed to amplify single loci. These 10 polymorphic loci will be useful for genotype identification and for estimation of genetic diversity in the hop collection maintained at the U.S. Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, Corvallis, Oregon. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
