ISHS Acta Horticulturae 668: I International Humulus Symposium
THE ELIMINATION OF VIRUSES AND HOP LATENT VIROID FROM HOP (HUMULUS LUPULUS L.) IN POLAND |
| Authors: | M. Grudzińska, E. Solarska |
| Keywords: | virus and viroid elimination, in vitro culture, meristem tip, hop plants, PNRSV, HpMV, HLVd |
| Abstract:
Hops in Poland are commonly infected with Hop latent viroid (HLVd) and viruses: Prunus necrotic ringspot virus (PNRSV) and Hop mosaic virus (HpMV). Previous experiments were successful in obtaining virus-free hop plants by heat therapy method but appeared to be inefficient for HLVd elimination. In these studies virus and viroid free hop plants were obtained by meristem-tip culture. The meristem tips were excised from lateral shoot tips of naturally infected plants and placed in vitro. In vitro regeneration ability varied between 0 and 46% of regenerated explants depending upon cultivar, depending upon the time at which meristems tips were obtained. The regenerated plants were tested by ELISA for the presence of viruses and by RT-PCR for the presence of HLVd. Plants with no detectable viruses and HLVd were used for further propagation. | |
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