ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION COMPETENCE OF DIFFERENT PRUNUS EXPLANTS, AS REVEALED BY GUS EXPRESSION |
| Authors: | D. Pratesi, R. Paris, P. Negri, A. Scotti |
| Keywords: | apricot, cherry, GUS assay, hormonal balances |
| Abstract:
In addition to morphogenic ability, competence to stable transformation (i.e. foreign DNA integration into chromosomes) is also essential in order to achieve transgenic plant regeneration. Transformation competence, as well as genotype and cell-type dependent factors, seems to be related to processes, such as DNA replication/ repair, cell de-differentiation and division, that can be strongly affected by explant preparation and culture conditions. This study, designed to set up efficient transformation protocols (i.e. favourable combinations of genotype, explant and culture conditions) for apricot and cherry, reports the most significant results of GUS histochemical assays performed on mature and zygotic tissues from one to more than twenty weeks after inoculation with A. tumefaciens (C58C1 pGV3850) carrying the p35SGUSIntron binary vector. The genotypes employed were ‘Sungiant’, ‘Portici’, ‘San Castrese’ and a clone of ‘Bella D'Imola’ (Cricot) for apricot, and ‘Colt’ (Prunus avium x pseudocerasus), ‘Lapins’ and ‘Celeste’ (P. avium) for cherry. Mature (leaf laminas and petioles) and zygotic (cotyledons) apricot explants were inoculated; for cherry genotypes, sections of in vitro etiolated shoots were studied including apices, leaf laminas, petioles and stem slices with or without axillary buds. The hormonal composition of the media was varied to affect callus proliferation from explant tissues, thought to be positively related to genetic transformation competence. | |
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