ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
SUITABILITY OF SELECTED STRAWBERRY CULTIVARS FOR GENOME MODIFICATION BY AGROBACTERIUM TUMEFACIENS |
| Authors: | A. Gruchała, M. Korbin, E. Żurawicz |
| Keywords: | Fragaria x ananassa, marker genes, transformation, regeneration |
| Abstract:
Twenty-five strawberry cultivars were analyzed to select genotypes most suitable for transformation. Four-week old leaf explants derived from in vitro culture were transformed with marker genes (construct pBIN19/nptII/gus) by Agrobacterium tumefaciens LBA4404 and regenerated on MS medium containing 1.5 mg L-1 BA, 0.1 mg L-1 IBA, 40 g L-1 glucose, 2 g L-1, and additionally 25 mg L-1 kanamycin and 300 mg L-1 of carbenicilin supplemented after two days of incubation. The number of regenerated shoots varied from 0.7 to 11 shoots per 100 transformed explants in comparison with 3.5-259.3 shoots from control tissues grown on medium without antibiotics. Transformation/regeneration efficiency, expressed as transformant number per 100 explants, was high for cultivars such as ‘Elista’ (9.5), ‘Wega’ (7.3), ‘Senga Precosa’ (6.5), ‘Kama’ (6.2), ‘Induka’ (4.5) and ‘Maria’ (3.0). ‘Redgauntlet’, ‘Zao Hang Guang’, ‘Dukat’ and ‘Favette’ seem to be unsuitable for genetic manipulation because of difficulties with their post-transformation regeneration (no regenerated shoots were observed under the described conditions). | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
