ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
IN VITRO MORPHOGENIC ABILITY OF MATURE OR EMBRYONIC APRICOT TISSUES |
| Authors: | R. Paris, D. Pratesi, P. Negri |
| Keywords: | Prunus armeniaca, shoot organogenesis, somatic embryogenesis, genotype, hormonal balance |
| Abstract:
A crucial point in the improvement of vegetatively propagated cultivars with gene transfer technologies is the availability of genotype-suitable methods for plant regeneration from mature tissues. Such protocols are still lacking for apricot (P. armeniaca L.), which can be most easily regenerated in vitro from seed explants. In this study, regeneration was attempted from mature tissues (leaves, petioles, internodes, roots, petals and stamen filaments) and seedling tissues (cotyledons) for several apricot cultivars. The explants were collected from either micropropagated shoots or field-grown plants and cultured on media supplemented with different combinations of auxins (NAA, IAA, IBA, 2,4-D) and cytokinins (BA, TDZ). A range of hormonal balances was tested to obtain either caulogenesis or somatic embryogenesis directly from explant tissues or through callus proliferation. Direct and indirect shoot organogenesis was efficiently obtained with mature tissues, mainly from the stipel axils of petioles. Somatic embryos sporadically differentiated from ‘Sungiant’ and ‘Portici’ leaf callus but did not develop into plants. Shoots and embryos differentiated from seedling material were able to complete their development in vitro. | |
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