ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
EMBRYO RESCUE OF CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.) |
| Authors: | M.J. Liu, Y.F. Qi |
| Keywords: | development pattern, in vitro culture, cold treatment, germination |
| Abstract:
Most cultivars of Chinese jujube (Ziziphus jujuba Mill.), a native fruit tree of China, have very serious embryo abortion. In order to get hybrids, embryo rescue of four excellent cultivars, ‘Dongzao’, ‘Jisifeng’, ‘Wudeng’ and ‘Fuzao’, was carried out. An optimized system for embryo culture was established. Firstly, young embryos were cultured in the germination medium: MS+BA 0.1~0.5 mg/L + IBA 0.5~1.0 mg/L + NAA 0.1 mg/L + Sucrose 50 g/L + Agar 7 g/L + LH 500 mg/L. Secondly, the regenerated seedlings were transferred to growing medium: MS + Sucrose 30 g/L + Agar 5 g/L. Three patterns of development of cultured embryos occurred with the increase of embryo age, i.e. continuous growth (embryo less than 30 days old), callus formation, or direct germination. Of the factors affecting embryo culture, the quality and age of the embryo were the most important. Low temperature treatment (2~4°C, 44 days) accelerated seedling formation but decreased the quality of seedling. 7~10 days dark treatment was helpful to rooting. Lateral roots of seedlings could be enhanced significantly by cutting the root tip. | |
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