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ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics

TRANSGENIC PLUMS - FRUIT-TREES RESISTANT TO SHARKA DISEASE

Authors:   M. Ravelonandro, R. Scorza
Keywords:   gene silencing, gene transfer, plum pox virus, Prunus domestica, virus resistance
Abstract:
Sharka is one of the most severe diseases damaging stone-fruit trees including apricot, cherry, peach and plum. The causative agent is plum pox potyvirus (PPV) that is naturally transmitted by aphids. No effective measures have been found to restrict PPV outbreaks and it has spread across European borders to the Middle East, North Africa, and North and South America. The difficulty in controlling PPV has led us to develop resistance through genetic engineering technology. The PPV capsid gene has been engineered into the plum genome, and a resistant clone has been identified. Posttranscriptional gene silencing (PTGS) is the mechanism involved in resistance. These resistant plants have been field tested under the appropriate permits and their ability to resist PPV infection in the long-term is being verified.

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