ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
DEVELOPMENT OF APRICOT MICROSATELLITES FOR CULTIVAR IDENTIFICATION |
| Authors: | J. Chaïb, L.S. Hagen, L. Krichen, P. Lambert, B. Fady, J.M. Audergon |
| Keywords: | Prunus armeniaca, SSR |
| Abstract:
Microsatellite markers (SSR) are powerful and efficient in characterising and identifying accessions in addition to their usefulness in molecular mapping and in phylogenetic studies. We have developed Prunus armeniaca L. SSR markers. A multiplex of five of these microsatellites was developed for use on an automatic sequencer. They were mapped onto a ‘Polonais’ x ‘Stark Early Orange’ hybrid progeny and applied to 118 genotypes from the germplasm collection, to principally derived clones (mutants and offspring of Bergeron and Goldrich cultivars) and to recent world-wide cultivars. The SSRs showed Mendelian inheritance and were mapped onto three different linkage groups. In the genetic diversity study, 86% of cultivars were easily identified. The informativity of SSR markers was thus illustrated in both cases. The multiplex of SSR markers offers a range of possibilities for cultivar protection, certification and genetic resource surveys of apricot. | |
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