ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
TRANSCRIPTOMICS AND PROTEOMICS TOOLS TOWARDS RIPENING MARKERS FOR ASSISTED SELECTION IN APRICOT |
| Authors: | J. Grimplet, C. Romieu, F.X. Sauvage, P. Lambert, J.M. Audergon, N. Terrier |
| Keywords: | Prunus armeniaca, EST, Isogenes |
| Abstract:
The many physicochemical changes that accompany apricot ripening (Prunus armenica) determine its organoleptic properties: increase of sugar/acid ratio, colour change, loss of firmness and synthesis of flavour compounds. These ripening related events also segregate in different cultivars. For a better knowledge of fruit ripening and to provide genomic tools for molecular breeding in apricot, we developed a systematic sequencing strategy mainly from 3' end of cDNAs. About 15000 EST were generated from three  zap cDNA libraries of apricots at three critical developmental stages (immature, half-ripe and ripe stages), yielding 5212 (35%) Unigenes.
The Unigene set was then compared with public databases to provide assignment to functional categories.
Three quarters (76%) of the Unigenes displayed homologies with public sequences, including 53% of known function proteins.
Most expressed categories were related to primary metabolism and stress response.
Electronic Northern Blot revealed strong activation of stress-related proteins and cell wall modifying enzymes during ripening.
Among the 1255 isoenzymes detected (amino-acid level similarity) in the Unigene set, 615 (49%) displayed significant homologies in their coding regions (nucleic acid level similarity). A proteomic approach was performed throughout apricot development to validate the electronic northern blots at a post-translational level and to find new ripening markers.
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