ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
A STRAWBERRY EST DATABASE FOR EVALUATING FRUIT QUALITY TRAITS AND SELECTING IMPROVED GENOTYPES THROUGH CDNA MICROARRAYS |
| Authors: | C. Rosati, F. Mourgues, F. Giorno, F. Carbone, M.A. Carboni, G. Perrotta |
| Keywords: | DNA chips, functional genomics, gene expression, ripening |
| Abstract:
To assess strawberry (Fragaria x ananassa Duch.) fruit quality and correlate gene expression data with those from biochemical analyses, we analyzed 3161 expressed sequence tags (ESTs) amplified from a fruit cDNA library. The 25 largest contigs related to plant genes with assigned functions comprised 499 ESTs (16% of the total), including genes not only associated with ripening (cell wall metabolism, sugar and acid synthesis, pigment formation, vitamin synthesis and allergenic properties), but also with plant-pathogen interactions, abiotic stresses and housekeeping functions. Over 1800 representative ESTs were used to produce cDNA microarrays to carry out comparative profiling experiments with genotypes of different fruit quality. Such microarray experiments have shown a number of genes whose differential expression correlated with differences among genotypes in important quality traits such as fruit firmness (cinnamoyl-CoA reductase, cinnamyl alcohol dehydrogenase, expansin, endo-  -1,4-glucanase) and volatile compound composition (alcohol acyl transferase).
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