ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
INVESTIGATION OF GENETIC VARIATION IN APPLE SEEDLING FAMILIES FOR RESISTANCE TO PHYTOPHTHORA CACTORUM USING DIFFERENT INOCULATION TECHNIQUES |
| Authors: | D.J. Smit, I.F. Labuschagné |
| Keywords: | Malus x domestica, leaf assay, fruit breeding, rootstock breeding, collar, crown and root rot |
| Abstract:
Against the background of varying approaches and results to quantify resistance to Phytophthora cactorum, we applied three different inoculation techniques on apple (Malus x domestica) seedling families in preliminary experiments to devise some procedure for reliable pre-screening. Results from mass drenching and leaf inoculation assays of seedling families indicate that the variation between families is at least partially genetic and therefore amenable to change by selection. A direct inoculation technique on intact shoots however, indicates contrasting reaction to inoculation, indicating that this technique should be revised. A one-shot mass selection among seedlings using the drenching method of inoculation should result in genetic response. The need to devise some procedure for re-testing selected seedlings, by inoculation of rooted cuttings rather than using excised shoot assays remains. | |
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