ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
TARGET SSR DEVELOPMENT IN PEACH AND SSR MAPPING IN A PEACH BC1 PROGENY |
| Authors: | I. Verde, E. Vendramin, M.T. Dettori, R. Quarta, Y. Wang, A.C. Lecouls, A.G. Abbott |
| Keywords: | microsatellite, Prunus persica, Prunus ferganensis, linkage map, BAC, molecular markers |
| Abstract:
Simple sequence repeats (SSRs) were developed in target regions of a peach linkage map using the peach bacterial artificial chromosome (BAC) resource available at Clemson University. The map was obtained using a BC1 progeny [peach x (peach x P. ferganensis)] and was composed of 109 loci covering 521 cM of the peach genome. Two regions of the map were chosen to develop SSRs. One was located in G5. This group was composed of only 4 loci covering 16 cM. An RFLP (FG202), localized on the homologous region of the anchored map (Texas x Earlygold), was chosen for this purpose. The other region was located in G7 and contained the QTL ‘powdery mildew resistance’. The RFLP AG104, closely linked to this QTL, was used. Of the five SSRs obtained, only one was polymorphic in our mapping population and localized on the targeted G7 region. A set of 24 SSRs previously obtained at Clemson University and which includes three SSRs linked to the evergrowing gene (evg), were also tested and mapped. In this way, an additional SSR was obtained for the QTL for mildew resistance, while evg could be approximately located on the distal part of G1b. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
