ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
THE RGA FAMILIY. PROMISING GENE ANALOG RELATED TO FIREBLIGHT AND SHARKA RESISTANCE IN PEAR AND APRICOT |
| Authors: | L. Dondini, F. Costa, L. Pierantoni, F. Gaiotti, R. Chiodini, S. Tartarini, S. Sansavini |
| Keywords: | Prunus armeniaca, Pyrus communis, Plum Pox Virus, Erwinia amylovora |
| Abstract:
Plant disease-resistance genes show highly conserved regions corresponding to characteristic amino acid domains which make it possible to isolate Resistance Gene Analogs (RGAs) with PCR using degenerate or heterologous primers. Putative RGAs were cloned and sequenced from the apricot Sharka resistant cultivar ‘Stark Early Orange’ and the pear fire blight tolerant cultivars ‘Harrow Sweet’, ‘US 309’ and ‘Old Home’). An AFLP-RGA technique (a modified-AFLP, with one of the two AFLP primers substituted by a primer designed on highly conserved resistance sequences) was also used in a pear progeny derived from the cross ‘Passe Crassane’ x ‘Harrow Sweet’ to identify putative disease resistance polymorphic markers. Apricot and pear RGAs showed a certain degree of homology and a subsequent gene bank database screening indicated that, in both species, they are closely related to at least one known resistance gene. Some RGA-markers identified appear to be related to Sharka and fire blight resistance, in apricot and pear. | |
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