ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
CLONING AND CHARACTERIZATION OF NBS RESISTANCE GENE CANDIDATE SEQUENCES IN ALMOND |
| Authors: | M. Martins, M.M. Oliveira |
| Keywords: | Fusicoccum amygdali, Prunus dulcis, RGC, NBS-LRR, molecular markers |
| Abstract:
A set of resistance-gene candidate (RGC) sequences were cloned from several cultivars of almond (Prunus dulcis), using a PCR-mediated approach with degenerated primers designed from conserved NBS (nucleotide binding site) motifs of known resistance genes (R-genes). ‘Primorskyi’, one of the cultivars used in this study is of particular interest since it is characterized as very tolerant to Fusicoccum amygdali, which causes a damaging and economically important disease in almond. Several of the cloned sequences could be translated into polypeptides without stop codons, and the sequences contained the characteristic motifs of the NBS-LRR class of plant disease R-genes. Cluster analysis carried out on almond NBS sequences, together with several analogous domains of known R-genes, indicated that almond sequences could be grouped in classes. Development of markers based on NBS sequences suitable for discriminating almond genotypes that are tolerant or susceptible to Fusicoccum amygdali can be evaluated in a near future based on this study. | |
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