ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
CLONING AND CHARACTERISATION OF NBS-LRR SEQUENCES IN APRICOT |
| Authors: | J.M. Soriano, S. Vilanova, C. Romero, G. Llácer, M.L. Badenes |
| Keywords: | NBS-LRR sequences, RGAs, Prunus armeniaca, disease resistance |
| Abstract:
Two combinations of degenerated primers from soybean, potato and sunflower, previously tested in peach, were used for obtaining NBS-LRR (Nucleotide Binding Site-Leucine Rich Repeats) sequences from two apricot cultivars resistant to sharka, ‘Goldrich’ and ‘Lito’, in order to identify RGAs (Resistance Gene Analogs) in apricot. Fifty clones per cultivar and primer combination were obtained which gave a total of 200 clones. The clones obtained were digested with five restriction enzymes. According to the restriction pattern, six and eight clone groups per primer combination were obtained from ‘Goldrich’ and ‘Lito’ respectively. Between 2 and 4 clones from each group were sequenced and compared with known resistance genes. Internal conserved motifs provided evidence that these sequences isolated from apricot may belong to the NBS-LRR gene family. A total of eighty-seven clone sequences obtained were aligned and grouped by cluster analysis into six families. A phylogenetic tree with these and other known RGA sequences was constructed. | |
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