ISHS Acta Horticulturae 663: XI Eucarpia Symposium on Fruit Breeding and Genetics
QTL ANALYSIS FOR RESISTANCE TO COLLETOTRICHUM ACUTATUM AND PHYTOPHTHORA CACTORUM IN OCTOPLOID STRAWBERRY (FRAGARIA X ANANASSA) |
| Authors: | B. Denoyes-Rothan, E. Lerceteau-Köhler, G. Guérin, S. Bosseur, J. Bariac, E. Martin, P. Roudeillac |
| Keywords: | anthracnose, Interval Mapping, linkage map, polyploid, pseudo-testcross |
| Abstract:
Colletotrichum acutatum and Phytophthora cactorum are two of the most important pathogens of strawberry in Europe, and particularly in France. The characterization of quantitative trait loci (QTL) controlling resistances to these two pathogens would allow a better knowledge of the genetic architecture of these resistances in order to develop durable resistance. A pseudo-testcross strawberry population derived from the cross between Capitola and CF1116 was used for detecting QTLs associated with C. acutatum and P. cactorum resistances. One hundred and eighty five individuals were inoculated with C. acutatum by dipping cold stored plants in a conidial suspension adjusted to 2.106 conidia per ml. They were also inoculated separately with P. cactorum by inserting an agar disk containing mycelium into the crown. Each inoculation was conducted twice. Putative QTLs for resistance to C. acutatum and P. cactorum were identified by Interval Mapping (Mapmaker-QTL) using a LOD threshold of 2. | |
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