ISHS Acta Horticulturae 662: VII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics
EMBRYO CULTURE STUDIES IN SUBTROPICAL PEACH (PRUNUS PERSICA BATSCH.) |
| Authors: | M. Srivastav, S.K. Singh, R.L. Arora, B. Krishna |
| Keywords: | peach, Prunus persica, embryo culture, subtropical peach, early maturity |
| Abstract:
A study on embryo culture of early maturing subtropical peach was carried out with the objectives to standardize the culture requirements for in vitro establishment, maturation and germination and to determine the embryo age for their in vitro germination. Sixty days aged in ovulo embryos of cultivar Flordasun were taken to standardize the cultural requirements during establishment/ maturation, germination and acclimatization, the effect of different media, organics, sucrose levels, growth regulators and photoperiods were studied. Embryos cultured in complete darkness on medium B5 (macro) + MS (micro and organics) supplemented with casein hydrolysate (200 mg l-1) + L-glutamine (200 mg l–1), sucrose (40 g l-1) and BAP (2.0 mg l-1) + IAA (0.5 mg l-1) showed better establishment and maturation. Germination was better for the embryos cultured on B5 (macro) + MS (micro and organics) supplemented with sucrose (30 g l-1) and BAP (1.5 mg l-1) + GA3 (1.0 mg l-1) under 12/12 h light and dark cycle. Ovules from two early maturing cultivars namely Saharanpur Prabhat and Flordasun were excised and cultured for determination of embryo age for their in vitro germination. In general, 60 days aged embryos of Saharanpur Prabhat and Flordasun, either open-pollinated or crossed with each other showed better culture establishment, maturation and germination than 70 and 80 days aged embryos. | |
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