ISHS Acta Horticulturae 662: VII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics
GENOTYPIC VARIATION AMONG STRAWBERRY CULTIVARS FOR SHOOT ORGANOGENESIS |
| Authors: | A.K. Singh, S.N. Pandey |
| Keywords: | BA, IBA, ms-medium, salts and vitamin, IAA, RH |
| Abstract:
Strawberry genotypes were evaluated for their competence to organogenesis. Adventitious shoots formed within 40 days of incubation in Pajaro and Sweet Charlie. Cultures were inducted in Murashige and Skoog Medium supplemented with growth regulators in several combinations. BAP (1 mg l-1) and IAA (1 mg l-1) in half strength MS medium produced highest number of shoots (13 per explant) in Pajaro. Sweet Charlie invariably responded slowly to growth regulator treatments in all the media tested. Highest number of explants (88%) of Pajaro produced shoots in culture. In regularly sub-cultured explants the frequency of transfer had no impact on further regeneration. Organogenesis from freshly inducted cultures was higher. The frequency of shoot production was higher in laterally bisected than those of intact leaves. In vitro shoots rooted easily in growth regulator free medium. Rooted plants were acclimatized in greenhouse with 90% survival. | |
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