ISHS Acta Horticulturae 662: VII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics
STUDIES ON SOMACLONAL VARIATION IN STRAWBERRY (FRAGARIA X ANANASSA DUCH.) CULTIVARS |
| Authors: | K. Kaushal, A.K. Nath, P. Kaundal, D.R. Sharma |
| Keywords: | strawberry, in vitro, chandler, BAP, NAA |
| Abstract:
Reproducible protocol for regeneration of complete plantlets from callus cultures using leaf and petiole explants have been standardized in cv. Chandler of strawberry. A combination of BAP (0.50 mg l-1) and NAA (0.75 mg l-1) was found to be best for induction and multiplication of callus cultures in cv. Chandler and Fern. It was 88.89% in cv. Chandler and 83.55% in cv. Fern. Plant regeneration was obtained only in cv. Chandler in MS medium containing a combination of BAP (2.0 mg l-1), NAA (0.25 mg l-1) and kinetin (0.5 mg l-1). In vitro, mutagenesis using gamma rays was carried out to induce variation. None of the four doses viz., 5, 10, 30 and 50 Gy were found to be lethal for calli. Axillary buds irradiated with lower doses of gamma rays (5 Gy) resulted in stimulatory effect on growth. A total of 7 variants were obtained on screening the somaclones and irradiated plantlets on the basis of phenotypic characters, fruiting characters, RAPD markers and resistance against leaf blight and root rot. In cv. Chandler, the frequency of variants was higher in somaclones (2.46%) than in irradiated plants (0.76%) obtained from axillary buds. The frequency of occurrence of the variants was found to be genotype dependent as it was higher in irradiated plants of cv. Fern (2.07%) than that of cv. Chandler (0.76%). | |
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