ISHS Acta Horticulturae 658: I International Symposium on Rootstocks for Deciduous Fruit Tree Species
MOLECULAR CHARACTERIZATION OF ROOTSTOCKS FOR SWEET CHERRY (PRUNUS AVIUM L.) |
| Authors: | A. Wünsch, R. Gella, J.I. Hormaza |
| Keywords: | SSRs, molecular markers, microsatellites, cultivar identification |
| Abstract:
Rootstock identification after grafting is very difficult following traditional characterization methods due to the absence of observable phenotypic traits. Consequently, the use of DNA-based markers can be useful to optimize the process of rootstock identification. Among those markers, microsatellites or SSRs are becoming the marker of choice for fingerprinting and genetic diversity studies in a wide range of living organisms. Thus, in this work, 9 microsatellites developed in peach have been used to identify 17 genotypes, which comprise several species and interspecific hybrids, useful as rootstocks for sweet cherry. The species studied include P. avium, P. cerasifera, P. cerasus, P. mahaleb, and the interspecific hybrids P. mahaleb x P. avium, P. avium x P. pseudocerasus, and P. cerasus x P. canescens. All the genotypes studied except one pair of siblings and one pair of accessions collected in the same location can be unequivocally distinguished with the combination of the 62 alleles obtained with the 9 polymorphic SSRs studied. UPGMA cluster analysis of the similarity data groups the genotypes studied according to the species to which they belong. | |
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