ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
NEW APPROACH IN THE IDENTIFICATION OF THE CAUSAL AGENT OF FIG MOSAIC DISEASE |
| Authors: | L. Serrano, J. Ramon, J. Segarra, V. Medina, M.A. Achón, M. López, M. Juárez |
| Keywords: | Incidence, virus, transmission, cytopathology, RT-PCR, Aceria ficus |
| Abstract:
Fig mosaic (FM) disease is transmitted by the eriophyid mite Aceria ficus, and is associated with the presence of double membrane-bound bodies (DMBs) in the cytoplasm of infected cells. The aetiology of FM disease is unknown although it has been related with several viruses. Epidemiological, biological and molecular studies have been conducted to determine the causal agent. Ultraestructural studies detected the DMBs and two different virus-like particles in FM leaf tissues. A 720 nm rod-shaped virus showing a tail of 230 nm was observed in virus-purified preparations and in FM susceptible herbaceous hosts. Two PCR products of 1.7 and 3.0 kbp were obtained from virus-purified RNA using universal primers of the family Potyviridae. Sequencing of these PCR products indicated that the genome is polyadenylated and not related with genera Rymovirus and Tritimovirus. The rod-shaped virus was also observed in the gut of mites but DMBs were not observed. In addition, a Poxviridae and/or Polydnaviridae-like virus was present in 80% of mites. | |
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