ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
DEVELOPMENT OF RT-PCR ASSAYS USING FLUOROGENIC 3' MINOR GROOVE BINDER DNA PROBES FOR DETECTION OF FRUIT TREE VIRUSES |
| Authors: | S. Marbot, J. Kummert, M. Salmon, M. Vendrame, O. Dutrecq, P. Lepoivre |
| Keywords: | ACLSV, ASPV, PDV, PNRSV, ApMV |
| Abstract:
In phytosanitary certification, there is currently a need for the development of reliable, sensitive and rapid tests for the routine detection of ilarviruses and latent viruses from fruit trees during the dormant season. We have here developed real-time RT-PCR assays that allow the reliable detection of Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem pitting virus (ASPV) in bark tissues of dormant wood. These assays are well adapted for the routine detection of these three viruses because they eliminate one risk of contamination by performing the whole test in a single closed tube. | |
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