ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
COMPARATIVE RESULTS BETWEEN DIFFERENT DETECTION METHODS OF VIRUS AND PHYTOPLASMAS FOR A PEAR AND APPLE CERTIFICATION PROGRAM |
| Authors: | A. Batlle, A. Laviña, M. García-Chapa, J. Sabaté, C. Folch, L. Asin |
| Abstract:
Plant certification and the introduction of new cultivars are key factors for increasing the market competitiveness of fruit companies. Some of the procedures and techniques used for virus and phytoplasma detection, following EPPO and UPOV protocols are time consuming and do not meet market demands, that require the introduction of new cultivars on a short-term basis. In this study different virus and phytoplasma detection methods are compared (i.e. field indexing, serological methods and PCR technique). In the detection of ASPV the results obtained were independent of the technique used, whereas CLSV was detected in a greater number of samples by ELISA compared to field indexing. Similarly ASGV was detected by ELISA in a tree, although no symptoms had been observed on the host indicator. The identification of phytoplasmas by indexing in woody hosts yielded no results in the first two years, while PCR technique detected the presence of Pear decline and Apple proliferation in some of the trees analyzed. | |
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