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ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases

INTERACTIONS BETWEEN CITRUS CACHEXIA VIROID AND CLOSELY RELATED SEQUENCE VARIANTS MAY IMPAIR EXPRESSION OF CACHEXIA SYMPTOMS

Authors:   A. Elleuch, M. Marrakchi, H. Fakhfakh, D. Lévesque, J.P. Perreault, N. Bessais
Keywords:   citrus, viroid, cachexia, HSVd, interference
Abstract:
We report the sequences of four variants of Hop stunt viroid (HSVd) isolated from a naturally infected citrus tree of the variety ‘Clementinier’ in Tunisia. One of the variants (HSVd Tun/cl3) had the characteristic changes of the cachexia-inducing variant CVd-IIb, whereas the others were more similar to variant CV-IIa not inducing cachexia. However, when material from this tree was bioassayed in the cachexia indicator ‘Parson’s special’, no symptoms were observed for two years after inoculation. This might result from the concurrent presence of non-cachexia inducing variants that could interfere with CVd-IIb. Our data also show the worldwide distribution of this viroid and the presence of mixture of sequence variants in natural infections.

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