ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
DETECTION OF PEAR BLISTER CANKER VIROID IN POME FRUIT ORCHARDS ACROSS AUSTRALIA |
| Authors: | P. Joyce, B. Rodoni, J.M. Crosslin, W.E. Howell, K.C. Eastwell |
| Keywords: | Pear blister canker viroid, molecular detection, RT-PCR, cloning |
| Abstract:
Pear blister canker viroid (PBCVd) has been detected in four cultivars of pears located in pome fruit districts across Australia. PBCVd has not previously been recorded in Australia and pear orchards do not show any typical or obvious symptoms of PBCVd infection. PBCVd was initially detected in a cultivar using dot-blot hybridisation as part of the phytosanitary requirements for the export of pome fruit budwood from Australia. As a result, a small survey was initiated and PBCVd was found to be widespread across Australia. A PBCVd specific RT-PCR confirmed the presence of this viroid in three pear cultivars. Nucleotide sequence obtained from cloned PCR products showed 97% homology to a published sequence variant of PBCVd (accession number Y18040). Initial results indicate variation between PBCVd isolates from different regions of Australia. Future plans are to obtain more sequence information and determine the origin of the PBCVd isolates in Australia. | |
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