ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
PLUM POX VIRUS IN TUNISIA: SOURCES AND PRELIMINARY CHARACTERISTICS |
| Authors: | M. Boulila, P. Briard, M. Ravelonandro |
| Keywords: | Tunisia, sharka virus, serology, molecular assays |
| Abstract:
Due to the upsurge of fruit tree germplasm exchange at the international level, Plum pox potyvirus (PPV) has moved from Europe to any other countries. Here, we report the first occurrence of PPV in Tunisia. Barks of suspicious Japanese plums were chip-budded onto GF 305 peach seedlings; crude extracts from plum leaves were inoculated to appropriate herbaceous indicators. Using the triple-antibody sandwich-enzyme-linked immunosorbent assay (TAS-ELISA) with the strain D specific-monoclonal antibodies, we have identified nine PPV isolates. The extension with the Immunocapture Reverse Transcription Polymerase Chain Reaction-based assay (IC/RT/PCR) permitted to amplify a 243 bp fragment by using the P1 and P2 primers. In an heminested second PCR cycle, using the nested primer PD, amplification resulted in a 198 bp fragment. RFLP analysis showed an AluI and a RsaI restriction sites in the PCR products. These analyses confirm that PPV strain D is occurred in Tunisia. | |
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