ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
APPLE CHLOROTIC LEAF SPOT VIRUS INFECTION INDUCES PLUM POX VIRUS-LIKE SYMPTOMS ON PLUM IN NEW ZEALAND |
| Authors: | B.S.M. Lebas, D.R. Elliott, R. vandenBrink, F.M. Ochoa-Corona, J.Z. Tang, B.J.R. Alexander |
| Keywords: | Plum pox virus, stone fruits, detection, survey |
| Abstract:
Fruit showing circular, sunken black necrotic lesions and circular ring symptoms from a single plum tree (Prunus domestica ‘Victoria’) from the Waikato region, North Island was submitted to Ministry of Agriculture and Forestry. Flexuous filamentous particles were observed by electron microscopy, suggesting the presence of Plum pox virus (PPV). However, the fruit tested negative for PPV and potyviruses by ELISA, RT-PCR, and IC-RT-PCR. Subsequently, RT-PCR and IC-RT-PCR for Apple chlorotic leaf spot virus (ACLSV) was performed and a positive result was obtained, confirming the observation of filamentous particles. The sunken necrotic lesions were consistent with infection by the bacterium Xanthomonas arboricola pv. pruni. Suspect samples collected within a 12 km radius of the initial site all tested negative for PPV. | |
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