ISHS Acta Horticulturae 657: XIX International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
BIOLOGICAL PROPERTIES AND PARTIAL MOLECULAR CHARACTERIZATION OF AN APRICOT STRAIN OF CGRMV |
| Authors: | D. Liberti, A. Ragozzino, P. Gentit, A. Marais, L. Svanella-Dumas, T. Candresse |
| Keywords: | Apricot, aetiology, double-stranded RNA, CGRMV |
| Abstract:
The initial purpose of this study was to investigate the aetiology of a degenerative disease of apricot originally noticed in the Campania region in 1985 (Ragozzino, 1991) and named “Apricot ring pox” (ARPox) from the symptoms observed in the initial host. The disease was later demonstrated to be graft transmissible on Apricot ‘Bulida’ and associated to the presence of Apple chlorotic leaf spot virus (ACLSV) (Ragozzino et al., 1992). Consistent with these observations, leaves of graft-inoculated GF305 indicators initially displayed typical ACLSV symptoms of dark green sunken mottle. However, at later times these indicators showed irregular ringspots and a marked clearing of the principal veins. To try to evaluate the possible presence of other viral agents, double stranded RNAs purified from the infected GF305 indicators were used as templates in a polyvalent PCR detection assay. Besides ACLSV, PCR products corresponding to two additional viral agents could be amplified and sequenced. One of the agents appears to be a new Trichovirus, while the other appears to represent a Foveavirus closely related to Cherry green ring mottle virus (CGRMV). A RT-PCR-based detection assay specific for CGRMV revealed the presence of this virus in another apricot accession showing symptoms of “Apricot ring pox”. Besides this initial association, further biological tests are clearly needed to evaluate the potential contribution of the apricot strain of CGRMV to the typical symptomatology of the disease. | |
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