List price   € 117     This title is available in ActaHort CD-rom format only - not available in print format Convener   G. Llácer  Editor   G. Llácer  Publication date   30 September 2004  ISBN   978-90-66051-48-5  ISSN   0567-7572  Number of articles   94  Volumes   1  Place   Valencia, Spain  Online articles: FOREWORD AND PREFACE (G. Llácer, ISHS Board of Directors) PRODUCTION AND USE OF POLYCLONAL ANTIBODIES TO THE COAT PROTEIN OF APPLE STEM GROOVING VIRUS EXPRESSED IN ESCHERICHIA COLI (O. Nickel, T.V. Fajardo, A.P. Trivilin, M.L.N.P. Targon, M.A. Machado) STUDIES ON THE INFLUENCE OF APPLE STEM GROOVING VIRUS ON TREE GROWTH OF VARIOUS APPLE CULTIVARS IN THE NURSERY (A. Maxim, L. Zagrai, I. Zagrai, M. Isac) APPLE STEM PITTING VIRUS DETECTION FROM DORMANT POME FRUITS BY RT-PCR (S. Paunović, D. Jevremović) APPLE VIRAL DISEASES DIAGNOSED IN THE GERMPLASM COLLECTION AT I.C.D.P. MARACINENI-ROMANIA (S. Popescu, G. Constantin, CR. Mazilu) A PRELIMINARY ACCOUNT OF THE PRESENCE OF POME FRUIT VIRUSES IN ALBANIA (A. Myrta, B. Di Terlizzi, B. Stamo, M.A. Rwahnih, V. Savino, L. Carraro) PRODUCTION OF MONOCLONAL ANTIBODIES FOR THE SEROLOGICAL IDENTIFICATION AND RELIABLE DETECTION OF APPLE STEM PITTING AND PEAR YELLOW VEIN VIRUSES IN APPLE AND PEAR (P. Gugerli, M.E. Ramel) COMPARATIVE RESULTS BETWEEN DIFFERENT DETECTION METHODS OF VIRUS AND PHYTOPLASMAS FOR A PEAR AND APPLE CERTIFICATION PROGRAM (A. Batlle, A. Laviña, M. García-Chapa, J. Sabaté, C. Folch, L. Asin) IDENTIFICATION OF A NEW TRICHOVIRUS IN SOME FRUIT TREES INFECTED BY APPLE CHLOROTIC LEAF SPOT VIRUS (ACLSV) (D. Liberti, A. Ragozzino, P. Gentit, A. Marais, L. Svanella-Dumas, T. Candresse) MOLECULAR CHARACTERIZATION OF A NEW FOVEAVIRUS IN PRUNUS ACCESSIONS OF ASIAN ORIGIN (A. Marais, L. Svanella-Dumas, X. Foissac, T. Candresse) DETECTION OF PLUM BARK NECROSIS STEM PITTING-ASSOCIATED VIRUS (PBNSPAV) FROM DIFFERENT STONE FRUIT SPECIES AND OPTIMISATION OF DIAGNOSTIC TOOLS (T. Amenduni, C. Hobeika, A. Minafra, V. Savino) NUCLEOTIDE SEQUENCE ANALYSIS AND DETECTION OF CHERRY RASP LEAF VIRUS (D. James) BIOLOGICAL PROPERTIES AND PARTIAL MOLECULAR CHARACTERIZATION OF AN APRICOT STRAIN OF CGRMV (D. Liberti, A. Ragozzino, P. Gentit, A. Marais, L. Svanella-Dumas, T. Candresse) VERTICAL TRANSMISSION OF PRUNUS NECROTIC RINGSPOT VIRUS BY GAMETES IN APRICOT (K. Amari, M.A. Sánchez-Pina, V. Pallás) THE OCCURRENCE AND MOLECULAR CHARACTERISATION OF PNRSV ISOLATES IN TURKEY (C. Ulubas, F. Ertunc) APPLE CHLOROTIC LEAF SPOT VIRUS INFECTION INDUCES PLUM POX VIRUS-LIKE SYMPTOMS ON PLUM IN NEW ZEALAND (B.S.M. Lebas, D.R. Elliott, R. vandenBrink, F.M. Ochoa-Corona, J.Z. Tang, B.J.R. Alexander) DETECTION OF SOME VIRUSES OF STONE FRUITS IN MOTHER PLANT BLOCKS IN EASTERN MEDITERRANEAN REGION OF TURKEY (G. Sertkaya, K. Caglayan, C. Ulubas) DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS IN APHID VECTORS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR (W.L. Schneider, D.J. Sherman, A.L. Stone, V.D. Damsteegt, R.D. Frederick) REAL-TIME RT-PCR OF PPV WITH R.A.P.I.D. A FIELD-HARDENED PCR UNIT FOR IN-FIELD DETECTION (V.A. Mavrodieva, L. Levy) REAL-TIME RT-PCR FOR QUANTITATIVE DETECTION OF PLUM POX VIRUS (A. Olmos, E. Bertolini, M. Gil, M. Cambra) DIRECT NESTED RT-PCR FOR THE DETECTION OF PLUM POX VIRUS (C. Varveri) FIRST DETECTION AND MOLECULAR CHARACTERISATION OF PPV-M STRAINS IN PLUM ORCHARDS IN SOUTH-WESTERN GERMANY (W. Jarausch, A. Baβler, N. Molla, G. Krczal) DETECTION AND CHARACTERIZATION OF PLUM POX VIRUS ISOLATES IN LOMBARDIA AND VENETO REGIONS (ITALY) (P.A. Bianco, I. Aliverti, P. Casati, G. Belli, S. Comes, A. Fanigliulo, A. Crescenzi) STRATEGY TO IDENTIFY AND TO CHARACTERIZE NEW ISOLATES OF PLUM POX VIRUS (M. Ravelonandro, P. Briard, M. Monsion) PRELIMINARY MOLECULAR CHARACTERIZATION OF PLUM POX POTYVIRUS ISOLATE W3174: EVIDENCE OF A NEW STRAIN (D. James, A. Varga) CHARACTERIZATION OF PLUM POX POTYVIRUS (PPV) BY DAS-ELISA AND RT-PCR/RFLP ANALYSIS IN TURKEY (K. Caglayan, G. Sertkaya, C. Ulubas, M. Kölber, L. Krizbai) PLUM POX VIRUS IN TUNISIA: SOURCES AND PRELIMINARY CHARACTERISTICS (M. Boulila, P. Briard, M. Ravelonandro) EPIDEMIOLOGY OF PLUM POX VIRUS IN JAPANESE PLUMS IN SPAIN (M. Cambra, M.T. Gorris, N. Capote, M. Asensio, M.C. Martínez, E. Bertolini, C. Collado, A. Hermoso de Mendoza, E. Mataix, A. López) POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION (V.D. Damsteegt, A.L. Stone, W.L. Schneider, D.G. Luster, F.E. Gildow) TRANSMISSION OF THREE NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS (F.E. Gildow, L. Levy, V.D. Damsteegt, A.L. Stone, W.L. Schneider, D.G. Luster) SPATIALLY ARRANGED DIVERSITY OF PLUM POX VIRUS (PPV) ISOLATES IN A CLUSTER OF ORCHARDS IN SOUTHERN FRANCE (V. Marie-Jeanne, G. Thébaud, J. Peyre, G. Labonne) APHID TRANSMISSION OF NATURAL RECOMBINANT PLUM POX VIRUS ISOLATES TO DIFFERENT PRUNUS SPP.-A CONTRIBUTION FOR UNDERSTANDING THE EPIDEMIOLOGY OF AN ATYPICAL PPV (M. Glasa, M. Boeglin, G. Labonne) RISK ASSESSMENT OF CONTAMINATION OF CHERRY TREES BY PLUM POX VIRUS IN FRANCE (M. Boeglin, J.B. Quiot, G. Labonne) SOUR CHERRY ISOLATE OF PLUM POX VIRUS (PPV-SOC): MONITORING OF VIRAL DISTRIBUTION IN INFECTED PRUNUS ROOTSTOCKS (M. Bodin, D. Verger, E. Costes, F. Dosba, M. Glasa) DETECTION AND ERADICATION OF PLUM POX VIRUS MARCUS TYPE IN ARAGÓN (SPAIN) (M.A. Cambra, J. Crespo, M.T. Gorris, M.C. Martínez, A. Olmos, N. Capote, M. Cambra) THE OCCURRENCE OF PPV IN CHERRY TREES IN THE CZECH REPUBLIC (M. Navrátil, D. Šafářová, A. Crescenzi, A. Fanigliulo, S. Comes, K. Petrzik, R. Kare¿ová) CURRENT STATUS OF SHARKA DISEASE IN SLOVENIA (M. Viršček, I. Mavrič) DETECTION OF PLUM POX POTYVIRUS IN WEEDS (M. Viršček, I. Mavrič, M. Urbančič-Zemljič, V. Škerlavaj) EVALUATION OF THREE ORNAMENTAL PRUNUS AS RESERVOIRS OF PPV (G. Labonne, M. Boeglin, B. Monsion) VARIABILITY IN SUSCEPTIBILITY TO PLUM POX VIRUS IN NATURAL WOODY HOSTS, MYROBALAN AND BLACKTHORN (J. Polák) RELATIONSHIP BETWEEN PLUM POX VIRUS AND PLUM BARK SPLIT VIRUS (ACLSV) IN PLUM (N. Minoiu, D. Vladianu, I. Oprean, M. Ravelonandro, P. Stegerean) IDENTIFICATION OF PLUM POX VIRUS AND SOME ILAR VIRUSES OF STONE FRUITS IN KAHRAMANMARAS DISTRICT OF TURKEY (E. Tolay Arikan, K. Caglayan, M.H. Gazel) RESISTANCE TO PLUM POX VIRUS: A MOLECULAR APPROACH (S. Vilanova, C. Romero, J.M. Soriano, J. Martínez-Calvo, G. Llácer, M.L. Badenes, J.D. Abernathy, A.G. Abbott) MOLECULAR GENETIC MAPPING OF THE PLUM POX VIRUS RESISTANCE GENES IN APRICOT (D. Abernathy, T. Zhebentyayeva, A.G. Abbott, S. Vilanova, M.L. Badenes, J. Salava, J. Polák, B. Krska, V.D. Damsteegt) UTILIZATION OF ALMOND AS SOURCE OF PLUM POX VIRUS RESISTANCE IN PEACH BREEDING (P. Martínez-Gómez, M. Rubio, F. Dicenta, T.M. Gradziel) LOCALIZATION OF COAT PROTEIN AND NUCLEIC ACID OF PLUM POX VIRUS IN PRUNUS PETIOLE AND STEM TISSUES (M. Rubio, B. Gosálvez, J.A. Hernández, F. Dicenta, P. Martínez-Gómez, J.A. Sánchez-Navarro, V. Pallás) IN SITU LOCALISATION OF PLUM POX PARTICLES IN SUSCEPTIBLE AND RESISTANT APRICOT CULTIVARS (L. Ion-Nagy, J.P. Eyquard, O. Legall, V. Decroocq, M. Hernould, J.P. Bru) EVALUATION OF THE SUSCEPTIBILITY OF SEEDLINGS FROM APRICOT CROSSES TO THE MARCUS STRAIN OF PLUM POX VIRUS (T. Amenduni, A. Bazzoni, A. Minafra, V. Savino) EVALUATION OF CHERRY CULTIVARS FOR THEIR RESPONSE TO INFECTION BY PLUM POX VIRUS SWEET CHERRY STRAIN (A. Fanigliulo, S. Comes, A. Crescenzi) ANALYSIS OF METHYLATION AND POST-TRANSCRIPTIONAL GENE SILENCING IN EMBRYOS AND SEEDLINGS OF C5, A TRANSGENIC PLUM POX VIRUS RESISTANT PLUM PRUNUS DOMESTICA L. (R. Scorza, K. Webb, M. Ravelonandro) POTENTIAL USE OF TRANSGENIC PLUMS RESISTANT TO PLUM POX VIRUS FIELD INFECTION (R. Scorza, T. Malinowski, N. Minoiu, M. Ravelonandro, M. Cambra) INVESTIGATIONS OF POTENTIAL ENVIRONMENTAL IMPACTS IN THE RELEASE OF TRANSGENIC PLUMS (M. Ravelonandro, N. Minoiu, R. Scorza) PRUNUS/PPV INTERACTIONS: A MOLECULAR APPROACH (V. Decroocq, V. Schurdi-Levraud, J.P. Eyquard, O. Legall, D. Wawrzyńczak, J. Del Amo, L. Simon, A Martínez) THE USE OF GREEN FLUORESCENT PROTEIN-TAGGED RECOMBINANT PLUM POX VIRUS (PPV) TO STUDY VIRAL INVASION IN PPV/PRUNUS INTERACTION (M. Lansac, J.P. Eyquard, O. Legall, V. Decroocq, V. Schurdi-Levraud, J.A. García) EXPRESSION OF SINGLE-CHAIN VARIABLE FRAGMENTS AGAINST STRUCTURAL AND NON-STRUCTURAL PROTEINS OF PLUM POX VIRUS IN TRANSGENIC NICOTIANA BENTHAMIANA PLANTS TO INTERFERE WITH VIRAL INFECTION (M. Gil, O. Esteban, M.C. Martínez, M.T. Gorris, L. Peña, M. Cambra, J.A. García) EFFECT OF PLUM POX VIRUS INFECTION ON PHOTOSYNTHESIS AND ANTIOXIDANT ENZYME ACTIVITY IN PEACH 'GF305' (J.A. Hernández, J.E. Olmos, B. Portillo, M. Rubio, F. Dicenta, P. Martínez-Gómez) EXPERIMENTAL EVIDENCE THAT APPLE DIMPLE FRUIT VIROID DOES NOT SPREAD NATURALLY (M. Malfitano, D. Alioto, A. Ragozzino, F. Di Serio, R. Flores) DETECTION OF APPLE SCAR SKIN VIROID IN APPLE TREES BY THE ISOTHERMAL NUCLEIC ACID AMPLIFICATION AND ELECTROCHEMILUMINESCENCE (H.R. Kim, S.H. Lee, J.S. Kim, M.S. Yiem, J.W. Park, C.K. Kim, J.D. Chung, Y.B. Shin) IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PEAR BLISTER CANKER VIROID ISOLATES IN CAMPANIA (SOUTHERN ITALY) (M. Malfitano, M. Barone, E. Ragozzino, D. Alioto, R. Flores) DETECTION OF PEAR BLISTER CANKER VIROID IN POME FRUIT ORCHARDS ACROSS AUSTRALIA (P. Joyce, B. Rodoni, J.M. Crosslin, W.E. Howell, K.C. Eastwell) DETECTION BY TISSUE PRINTING OF STONE FRUIT VIROIDS, FROM EUROPE, THE MEDITERRANEAN AND NORTH AND SOUTH AMERICA (H. Torres, G. Gómez, V. Pallás, B. Stamo, A. Shalaby, B. Aouane, I. Gavriel, P. Kominek, K. Caglayan, M. Sipahioglu, R. Michelutti, A. Myrta, N. Fiore) MOLECULAR CHARACTERIZATION OF HOP STUNT VIROID (HSVD) SEQUENCE VARIANTS FROM PRUNUS SPECIES (E. Ragozzino, F. Faggioli, M. Barba, M. Malfitano) DEVELOPMENT OF A PCR METHOD OF PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID DETECTION FOR CERTIFICATION OF PLANTING MATERIAL (M. Hassan, M. Zouhar, P. Rysanek) MIXED INFECTION OF PEACH TREES WITH PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID IN THE CZECH REPUBLIC (M. Hassan, P. Rysanek, F. Di Serio) INTERACTIONS BETWEEN CITRUS CACHEXIA VIROID AND CLOSELY RELATED SEQUENCE VARIANTS MAY IMPAIR EXPRESSION OF CACHEXIA SYMPTOMS (A. Elleuch, M. Marrakchi, H. Fakhfakh, D. Lévesque, J.P. Perreault, N. Bessais) OVERWINTERING ADULTS AND SPRINGTIME GENERATION OF CACOPSYLLA PICTA (SYNONYM C. COSTALIS) CAN TRANSMIT APPLE PROLIFERATION PHYTOPLASMAS (B. Jarausch, N. Schwind, W. Jarausch, G. Krczal) ESTABLISHMENT OF A QUANTITATIVE REAL-TIME PCR ASSAY FOR THE QUANTIFICATION OF APPLE PROLIFERATION PHYTOPLASMAS IN PLANTS AND INSECTS (W. Jarausch, T. Peccerella, N. Schwind, B. Jarausch, G. Krczal) ANALYSIS OF THE DISTRIBUTION OF APPLE PROLIFERATION PHYTOPLASMA SUBTYPES IN A LOCAL FRUIT GROWING REGION IN SOUTHWEST GERMANY (W. Jarausch, N. Schwind, B. Jarausch, G. Krczal) GENETIC VARIABILITY OF AP PHYTOPLASMAS DETECTED IN THE APPLE GROWING AREA OF TRENTINO (NORTH ITALY) (C. Cainelli, C. Bisognin, M.E. Vindimian, M.S. Grando) PEAR DECLINE PHYTOPLASMA DETECTION IN PEAR TREES AND INSECT VECTORS BY DOT-BLOT HYBRIDIZATION AND NESTED-PCR (M. García-Chapa, A. Batlle, A. Laviña, L. Galipienso, G. Firrao) TOLERANCE INCREASE TO PEAR DECLINE PHYTOPLASMA IN MYCORRHIZAL OHF-333 PEAR ROOTSTOCK (M. García-Chapa, A. Batlle, A. Laviña, A. Camprubí, V. Estaún, C. Calvet) IDENTIFICATION OF PHYTOPLASMAS ON POMACEOUS FRUIT TREE SPECIES IN HUNGARY (I. Ember, M. Nemeth, L. Krizbai, M. Kolber, M. Szakál, S. Botti, A. Bertaccini, GY. Bohár, R. Zsovák-Hangyál) TRANSMISSION OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA TO PRUNUS SPECIES BY USING VECTOR AND GRAFT TRANSMISSION (L. Carraro, F. Ferrini, P. Ermacora, N. Loi) DISTRIBUTION OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA IN APRICOT TREES IN WESTERN SWITZERLAND (M. Genini, M.E. Ramel) EPIDEMIOLOGICAL SURVEY OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA IN TWO ORCHARDS IN WESTERN SWITZERLAND (M.E. Ramel, P. Gugerli) DATA ON THE LIFE CYCLE OF CACOPSYLLA PRUNI, PSYLLIDAE VECTOR OF EUROPEAN STONE FRUIT YELLOWS (ESFY) PHYTOPLASMA, IN FRANCE (G. Labonne, J. Lichou) SPATIO-TEMPORAL ANALYSIS OF DISEASE SPREAD PROVIDES INSIGHTS INTO THE EPIDEMIOLOGY OF EUROPEAN STONE FRUIT YELLOWS (G. Thébaud, C. Castelain, G. Labonne, J. Chadoeuf) SURVIVAL OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA OUTSIDE FRUIT CROP PRODUCTION AREAS: A CASE STUDY IN SOUTHEASTERN FRANCE (M. Yvon, G. Labonne, G. Thébaud) EPIDEMIOLOGY OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA IN THE CZECH REPUBLIC (R. Fialová, M. Navrátil, P. Válová, P. Lauterer, F. Kocourek, Z. Poncarová-Voráčková) OCCURRENCE AND EPIDEMIOLOGY OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA IN SPAIN (A. Laviña, J. Sabaté, M. García-Chapa, A. Batlle, E. Torres) IMPROVED MOLECULAR METHODS FOR DETECTION OF EUROPEAN STONE FRUIT YELLOWS (ESFY) PHYTOPLASMAS FROM IN VITRO SHOOTS OF FRUIT TREES (A. Bertaccini, S. Paltrinieri, L. Caprara, M. Laimer, V. Hanzer, I. Balla) THREE YEARS OF MOLECULAR MONITORING OF PHYTOPLASMA SPREADING IN A PLUM GROWING AREA IN ITALY (S. Paltrinieri, A. Bertaccini, A. Lugli, W. Monari) PHYTOPLASMA DETECTION IN EMPOASCA DECEDENS AND EMPOASCA SPP. AND THEIR POSSIBLE ROLE AS VECTORS OF EUROPEAN STONE FRUIT YELLOWS (16SRX-B) PHYTOPLASMA (M. Pastore, E. Raffone, M. Santonastaso, R. Priore, S. Paltrinieri, A. Bertaccini, A.M. Simeone) DETECTION OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA (ESFYP) IN HOMOPTERA INSECTS AND IN WILD STONE FRUIT TREES COLLECTED IN PEACH ORCHARDS IN NORTHERN ITALY (C.P. Pollini, R. Bissani, L. Giunchedi, N. Mori, D. Dradi, T. Visigalli) FIRST DETECTION OF EUROPEAN STONE FRUIT YELLOWS PHYTOPLASMA (ESFY) IN BELGIUM (T. Olivier, J. Kummert, S. Steyer) PHYTOPLASMA DISEASES OF STONE FRUIT TREES IN POLAND (M. Cieślińska, H. Morgaś, T. Jakubowski) PHYLOGENETICAL CHARACTERIZATION AND PCR DETECTION OF A NEW PHYTOPLASMA IN ALMOND (PRUNUS AMYGDALUS) AND PEACH (PRUNUS PERSICAE) IN THE MEDITERRANEAN AREA (E. Verdin, P. Salar, P. Danet, J.L. Danet, B. Gélie, J.M. Bové, M. Garnier, E. Choueiri, F. Jreijiri, S. El Zammar) OCCURRENCE OF PSYLLIDS IN APPLE ORCHARDS AND TRANSMISSION OF APPLE PROLIFERATION BY CACOPSYLLA PICTA (SYN. C. COSTALIS) IN GERMANY (E. Seemüller, E. Dickler, C. Berwarth, W. Jelkmann) SAMPLING PROTOCOLS AND RISK OF ERROR SIGNIFICANCE IN MOLECULAR DETECTION TESTS FOR FRUIT TREES CERTIFICATION (J. Kummert, M. Malice, S. Marbot, P. Lepoivre, S. Steyer, R. Oger) DEVELOPMENT OF RT-PCR ASSAYS USING FLUOROGENIC 3' MINOR GROOVE BINDER DNA PROBES FOR DETECTION OF FRUIT TREE VIRUSES (S. Marbot, J. Kummert, M. Salmon, M. Vendrame, O. Dutrecq, P. Lepoivre) MULTIPLEX DETECTION OF PLANT (QUARANTINE) PATHOGENS BY MICRO-ARRAYS: AN INNOVATIVE TOOL FOR PLANT HEALTH MANAGEMENT (C.D. Schoen, M. de Weerdt, M. Szemes, P. Bonants, R. van Beuningen, A. Chan, R. Hilhorst, P. Boender) NEW APPROACH IN THE IDENTIFICATION OF THE CAUSAL AGENT OF FIG MOSAIC DISEASE (L. Serrano, J. Ramon, J. Segarra, V. Medina, M.A. Achón, M. López, M. Juárez) IDENTIFICATION OF CITRUS TRISTEZA VIRUS STRAINS IN LOUISIANA AND ALABAMA (R. Valverde, A. Landry, M. Nesbitt, P. Lotrakul, W. Dozier, R. Ebel) INTERNATIONAL WORKING GROUP ON FRUIT TREE VIRUSES (W. Jelkmann) GUIDELINES FOR THE DEVELOPMENT OF NEW METHODS FOR VIRUS TESTING OF FRUIT TREES AND SMALL FRUIT CROPS (R.R. Martin, W. Jelkmann, T. Candresse)