ISHS Acta Horticulturae 656: X International Symposium on Small Fruit Virus Diseases
OCCURRENCE OF PHYTOPLASMAS IN RED AND WHITE CURRANT IN THE CZECH REPUBLIC |
| Authors: | M. Navrátil, P. Válová, R. Fialová, R. Karešová, J. Přibylová, J. Špak, D. Kubelková |
| Keywords: | Ribes rubrum, European stone fruit yellows phytoplasma, apple proliferation phytoplasma, pear decline phytoplasma, aster yellows phytoplasma, PCR, RFLP, TEM, sequence analysis |
| Abstract:
The presence of phytoplasmas in the shrubs of red ('Vitan', 'Losan', 'Mulka', 'Rubigo', and some of new selections) and white ('Primus') currant exhibiting full blossom symptoms was estimated using nested PCR with primers amplifying phytoplasma 16S rRNA gene and by electron microscopy. In total, 20 of 41 analysed symptomatic bushes tested positive phytoplasma in PCR. Based on the results of RFLP analysis and sequencing the phytoplasma isolates were identified as belonging to the apple proliferation and aster yellows groups. European stone fruit yellows phytoplasma was the most common phytoplasma identified in currants in this study. Furthermore, apple proliferation phytoplasma, aster yellows phytoplasma and pear decline phytoplasma were detected. The presence of phytoplasma bodies in phloem tissue of red currants was confirmed by electron microscopy. | |
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