ISHS Acta Horticulturae 653: IX International Symposium on Plant Bioregulators in Fruit Production
6-BENZYLADENINE AND THIDIAZURON INDUCED SPECIFIC GENES IN ORGANOGENESIS OF 'WHANGKEUMBAE' PEAR (PYRUS PYRIFOLIA) |
| Authors: | C.S. Kim, C.H. Lee, G.P. Lee |
| Keywords: | suppression subtractive hybridization (SSH), BA, TDZ, photolyase, carbonic anhydrase, serine proteinase inhibitors, ACC synthase, serine/threonine protein kinase, RING Finger Proteins, diminuto |
| Abstract:
For screening the effect of BA and TDZ on leaf explant in vitro of Pyrus pyrifolia, the suppression subtractive hybridization (SSH), which is an improved method of mRNA-differential display based on PCR procedure, was applied. BA treatment induced 13 novel and unique mRNAs on leaf explants in Pyrus pyrifolia cv. Whangkeumbae. mRNA of metallothioneins (MT) was induced by BA treatment, where generally the induction of MT in plant reflects the increased concentration of metal ions in cytosols. BA treatment also affected stress condition and DNA repair system in this plant. The mRNAs of light-harvesting chlorophyll a/b binding (LHC) proteins, photolyase, carbonic anhydrase, serine proteinase inhibitors (serpins) were also induced. BA treatment generally showed rapid cell growth and division, so mRNAs of lipid-transfer proteins (LTPs) and hemoglobins were largely induced. TDZ treatment generated 11 specific mRNAs. TDZ treatment like other cytokinins resulted in rapid cellular development/division, in entering stress condition, and in inducing repair system, so related mRNAs such as those of ribosomal protein L16, chaperonin 10 (Cpn10), chlorophyll a/b binding protein, 1-aminocyclopropane-1-carboxylate (ACC) synthase, serine/threonine protein kinase, RING finger proteins, and ubiquitin fusion protein were induced. Moreover, mRNAs of putative receptor-like protein kinase, a hypothetical protein, which is novel and putatively related to signal transduction in cellular level, were largely induced. Interestingly, mRNA of diminuto (a dwarf gene) was also induced; this would be related to the physiological data showing the stunt and dwarf shoot regeneration. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
