ISHS Acta Horticulturae 652: I International Symposium on Grapevine Growing, Commerce and Research
GENETIC TRANSFORMATION OF ROOTSTOCK CULTIVAR RICHTER 110 WITH THE GENE ENCODING THE IRONBINDING PROTEIN, FERRITIN |
| Authors: | R. Oláh, A. Tóth, S. Ruthner, J. Korbuly, E. Szegedi |
| Keywords: | thidiazuron (TDZ), benzyladenine (BA), somatic embryogenesis, Vitis, Agrobacterium tumefaciens, nptII gene, grapevine |
| Abstract:
Molecular breeding methods have recently been widely applied to improve the quality, stress- and disease resistance of grapevine cultivars. Our genetic transformation experiments started ten months ago aiming to obtain stress tolerant grapevine plants by using the gene encoding the ironbinding protein, ferritin, cloned from Medicago sativa. Involvement of ferritin in the oxidative stress response is supported. Therefore, it seems that the overexpression of ferritin in grapevine plants makes them tolerant to oxidative damage and pathogen attack. Somatic embryos (with size of 1-2 mm) of Richter 110 were treated with Agrobacterium tumefaciens EHA101(pRok2::Ferr). The germinating embryos were transferred on light three months ago and the embryos developed to green plantlets. The plant regeneration is in process at the present time and the small plantlets show normal development on the selective medium. | |
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