ISHS Acta Horticulturae 652: I International Symposium on Grapevine Growing, Commerce and Research
ISOENZYME POLYMORPHISM OF SOME GRAPEVINE (VITIS VINIFERA L.) |
| Authors: | G.G. Jahnke, J. Májer, J. Korbuly |
| Keywords: | isoenzyme, cultivar identification, molecular markers, PAGE |
| Abstract:
Isoenzyme analysis was used for the investigation of genetic variability of 20 grapevine (Vitis vinifera L.) cultivars. Vertical poliacrylamide-gel electrophoresis was employed to investigate the soluble isoenzymes of catechol-oxidase (CO), peroxidase (PER), esterase (EST), acid phosphatase (AcP), Leucine aminopeptidase (LAP), Glutamate-oxalacetate transaminase (GOT), Glucose-phosphate isomerase (GPI) or Phosphoglucomutase (PGM). We have established that the isoenzymatic characterisation of grapevine cultivars, with the combination of different enzyme systems, is suitable for the identification the cultivars. | |
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