ISHS Acta Horticulturae 651: XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals - Part II
THE USE OF CHROMOSOMAL MARKERS LINKED WITH NUCLEOLI ORGANISERS FOR F1 HYBRID VERIFICATION IN LILIUM |
| Authors: | A. Marasek, T. Orlikowska, R. Hasterok |
| Keywords: | 25S rDNA, FISH, interspecific hybrids, lily, NOR, silver staining |
| Abstract:
In this study, chromosomal markers based on the presence and position of secondary constrictions, connected with the nucleoli organising regions (NORs), were described with the goal to use them for verification of the hybrid status of F1 lily plants obtained from crosses ‘Marco Polo’ x Lilium henryi and ‘Expression’ x L. henryi. Two methods of differentiation of secondary constrictions were used – silver staining, which detects some nucleolus-linked proteins and can be used to determine expression of 18S-5.8S-25S rRNA genes and in situ hybridisation with a 25S rDNA probe, which reveals the chromosomal localisation of these genes regardless of their transcriptional status. These two techniques allowed identifying 4 chromosomes in L. henryi and 5 chromosomes in ‘Expression’ out of the complement of 24 chromosomes. In ‘Marco Polo’ 5 chromosomes were recognisable after silver staining and 6 ones using in situ hybridisation with 25S rDNA probe. The two techniques also revealed some polymorphism regarding both the number of hybridisation sites and Ag-positive bands observed in ‘Marco Polo’ and ‘Expression’. For hybrid verification only chromosomes carrying non-polymorphic landmarks were chosen: chromosomes D and K for cultivars and chromosomes F for L. henryi. | |
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