ISHS Acta Horticulturae 651: XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals - Part II
INTERSPECIFIC HYBRIDISATION IN FLOWERING SHRUBS |
| Authors: | J. Van Huylenbroeck, K. Van Laere, T. Eeckhaut, E. Van Bockstaele, E. Van Bockstaele |
| Keywords: | embryo rescue, Hydrangea, incongruity, in vitro, Ligustrum, ornamentals |
| Abstract:
Interspecific crosses within the genera Ligustrum and Hydrangea were made to introduce new genetic variation. Ploidy determinations and pollen tube staining revealed no prezygotic barriers within the gene pool used. Green and vigorous Hydrangea seedlings could only be obtained from crosses in which H. paniculata was used as seed parent. The reciprocal crosses did not yield germinating ovules. H. paniculata x H. quercifolia seedlings showed an introgression of paternal AFLP-markers. In Ligustrum, abortion rates were very high except for crosses within the evergreen section. Germination of ovules was only achieved after fruits had developed on the seed parent for 11 weeks. Introgression rates of paternal DNA-sequences are divergent; only crosses between L. japonicum and L. lucidum were found to be true hybrids. | |
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