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ISHS Acta Horticulturae 650: I International Symposium on Saffron Biology and Biotechnology

IMPROVED CONDITIONS TO EXTRACT EXOGENOUS YELLOW WATER-SOLUBLE COLORANTS ADDED TO SAFFRON AVOIDING CROCETIN ESTERS INTERFERENCES

Authors:   A. Zalacaín, I. Blázquez, E.M. Díaz-Plaza, M. Carmona, G.L. Alonso
Keywords:   adulteration, annato, hydrolysis, naphtol yellow, quinoleine yellow, sunset yellow, tartracine
Abstract:
Saffron samples from 4 different production countries were extracted for 10 minutes. The extract was then submitted to different conditions of time (0, 5, 10, 15 min), temperature (RT, 60 and 90 ºC) and pH (2.0, 1.0, 0.1). Different solutions of yellow colorants (naphtol yellow, quinoleine yellow, annato, tartracine and sunset yellow) were assayed at these conditions. The degradation of crocetin esters and colorants was followed by UV-Vis spectrometry. At pH extract of 0.1 and 10 min at 90 ºC, the crocetin esters disappear and no colorants were affected.

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