ISHS Acta Horticulturae 650: I International Symposium on Saffron Biology and Biotechnology
THE MORPHOLOGICAL STUDY OF AMYLOPLAST DISTRIBUTION IN CROCUS SATIVUS L. FIBROUS ROOTS |
| Authors: | B. Behboodi, L. Samadi |
| Keywords: | amyloplasts, PAS staining |
| Abstract:
The fibrous roots of Crocus sativus L. emerge from a single ring of its corms and these roots are not branched. We studied the distribution of amyloplasts in these roots by using periodic acid Shiff staining (PAS). As the result of PAS staining the amyloplasts are colored purple. Four morphogenic regions can be distinguished in proliferation region of a fibrous root: Cap, Meristem, Cortex initiation, and central cylinder initiation. Results of PAS staining shows that the amyloplasts are stored in cap cells, mostly. The amyloplasts were partly observed in meristem, cortex and central cylinder as well. The size of amyloplasts in cap cells is larger than amyloplasts of cortex and central cylinder initiation. There is no amyloplast in caliptra and meristem cells which evident that the formation of these grains may occur during differentiating stages. | |
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