ISHS Acta Horticulturae 650: I International Symposium on Saffron Biology and Biotechnology
PLANTLET REGENERATION VIA SOMATIC EMBRYOGENESIS IN FOUR SPECIES OF CROCUS |
| Author: | R. Karamian |
| Keywords: | meristems, micropropagation, plant growth regulators, somatic embryos |
| Abstract:
Somatic embryogenesis was initiated in four species of Crocus, C. sativus, C. cancellatus, C. michelsonii and C. caspius using shoot meristem culture on LS medium containing 4 mg/l NAA and 4 mg/l BA or 1 mg/l 2,4-D and 4 mg/l kinetin. Somatic embryogenesis was asynchronous in all of four species and various stages of somatic embryo development were observed when embryogenic calli with globular somatic embryos were transferred into half strength MS medium containing 1 mg/l abscisic acid. Maturated embryos could be germinated on half strength MS medium supplemented with 25 mg/l GA3. Complete plantlets were obtained by transferring of germinated embryos into half strength MS medium supplemented with 1 mg/l NAA and 1 mg/l BA at 20 ºC under a 16/8 h (light/dark) cycle. | |
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