ISHS Acta Horticulturae 649: Euro Berry Symposium - Cost 836 Final Workshop
CRYOPRESERVATION OF IN VITRO APICES OF STRAWBERRY (FRAGARIA X ANANASSA DUCH.) BY A NEW ENCAPSULATION-DEHYDRATION METHOD |
| Authors: | C. Ramírez, J.M. López-Aranda, J. Gálvez, C. Soria, J.F. Sánchez-Sevilla, J.J. Medina, M.E. Gónzalez-Benito |
| Keywords: | meristems, long-term conservation, cold-hardening, germplasm, liquid nitrogen, silica gel. |
| Abstract:
Cryopreservation of shoots apices of strawberry by encapsulation-dehydration method has been successfully carried out in some cultivars (Clavero-Ramírez et al., 2001). However, other cultivars have produced low viability with the same method. A new protocol of encapsulation-dehydration (Sakai et al., 2000) has been used to improve shoot formation rate of these cultivars. In this new protocol, the apices are encapsulated in a medium with glycerol and the pre-treatment with high sucrose concentration, required by conventional procedure, is eliminated. We present the preliminary studies carried out using this new method with several strawberry cultivars (‘Andana’, ‘Camarosa’, ‘Carisma’) and a wild genotype (F. virginiana). | |
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