ISHS Acta Horticulturae 649: Euro Berry Symposium - Cost 836 Final Workshop
IN VITRO STORAGE OF BERRY GENOTYPES |
| Authors: | M. Coman, V. Isac, P. Mladin, A. Popescu |
| Keywords: | Fragaria, Rubus, slow growth culture, cold storage, genetic resources |
| Abstract:
In vitro maintenance of small fruit germplasm under cold storage conditions was initiated at the Fruit Research Institute in the 1980s. Especially in recent years this was done according to the principles of an in vitro active genebank, all material flowing through a cyclical process of multiplication and monitoring for stability. Long term storage of strawberry germplasm at low temperature is currently achieved on Lee & Fossard basic medium lacking any growth regulators. For raspberries and blackberries, the in vitro maintenance of germplasm under cold storage conditions is currently achieved on Murashige & Skoog medium, supplemented with small quantities of IBA (in raspberry), NAA (in blackberry), BA and GA3. At 4ºC, under sub-optimal conditions for in vitro growing, the time interval for subculturing event was established at about 3-4 months for raspberries, 4-6 months for blackberries, and 8 months to one year for strawberries, respectively. The in vitro storage of the small fruit germplasm could be achieved without any difficulties, the loss of material being only accidental. Moreover, there is no evidence of genetic instability among the plants obtained from the microshoots preserved in vitro under cold storage conditions. So far, the number of in vitro preserved Fragaria and Rubus genotypes was essentially limited to the needs for commercial micropropagation. At this moment, a total of 124 genotypes are maintained under cold storage at 4ºC as in vitro microshoots, including 43 cultivars and clones of Fragaria x ananassa, 5 clones of F. vesca, 3 clones of F. virginiana, one cultivar of F. moschata, one cultivar of F. x vescana, six Fragaria interspecific hybrids, 21 cultivars and 11 clones of Rubus idaeus, 23 cultivars of R. procerus, one clone of R. occidentalis, and one clone of R. phenicolasius. | |
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