ISHS Acta Horticulturae 649: Euro Berry Symposium - Cost 836 Final Workshop
CROSS-SPECIES AMPLIFICATION AND PHYLOGENETIC RECONSTRUCTION USING FRAGARIA MICROSATELLITE PRIMERS |
| Authors: | D.J. Sargent, A.M. Hadonou, M.J. Wilkinson, N.H. Battey, J.A. Hawkins, D.W. Simpson |
| Keywords: | systematics, phylogeny, SSR, transferability, strawberry |
| Abstract:
Twenty-eight microsatellite primer pairs developed from Fragaria vesca ‘Rügen’ were applied to sixteen accessions representing eight diploid Fragaria species. The number of alleles generated, the power of discrimination and the percentage of accessions where no PCR product could be amplified were calculated for each locus for the thirteen non-F. vesca accessions. A phylogeny was then generated for the species accessions sampled, using the presence or absence of alleles at the polymorphic loci as character states. Despite the problems inherent in phylogeny reconstruction from microsatellite data, the phylogeny showed some congruence with a previously published phylogeny of Fragaria, based on nucleotide sequence data. However, relationships inferred from microsatellite allele data were relatively unresolved and poorly supported. The genetic basis of allelic polymorphisms at specific loci was investigated through direct sequencing of the PCR products amplified by three primer pairs. The potential utility of sequence data generated from microsatellite loci in evolutionary studies of closely related species groups is briefly explored. | |
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