ISHS Acta Horticulturae 640: XXVI International Horticultural Congress: Viticulture - Living with Limitations
TISSUE CULTURE IMPROVES THE PROPAGATION OF 'NORTON' GRAPEVINE (VITIS AESTIVALIS) |
| Authors: | B.B. Bigger, P.E. Read |
| Keywords: | acclimation, in vitro, ex vitro, cytokinin, auxin, peat pellet, sundae cup |
| Abstract:
Propagation of the Vitis aestivalis cultivar ‘Norton’ (syn. ‘Cynthiana’) through traditional woody cuttings has been difficult. Rooting of woody cuttings has been a major hindrance in propagating this cultivar and providing enough plants to meet grower needs. In vitro propagation offers another method of increasing plant material. Cultures were established and maintained on MS medium supplemented with 4 μM 6-benzyladenine (BA) and 0.5 mg/L thiamine and solidified with 7.5 g/L Bacto-Agar. The objectives of this study were to determine optimal methods for in vitro production and ex vitro establishment of ‘Norton’ plantlets. A factorial combination of four concentrations of BA (0, 2, 4 and 8 μM) and two concentrations of naphthaleneacetic acid (NAA) (0 and 0.5 μM) was used for the multiplication study. Plantlets were acclimated to ex vitro conditions without in vitro rooting. Plantlets were rooted ex vitro either with or without a 1000 ppm (0.1%) indolebutyric acid (IBA) dip for 5 seconds. Auxin did not have an effect on explant growth or plantlet rooting. Propagation of Norton through tissue culture appears to have potential to provide more plant material for growers in an expeditious manner. | |
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