ISHS Acta Horticulturae 640: XXVI International Horticultural Congress: Viticulture - Living with Limitations
USE OF RAPD MARKERS TO IDENTIFY FRUIT COLOR IN GRAPEVINE: FIRST RESULTS |
| Authors: | M.L. Miaja, R. Vallania, R. Caramiello, A. Akkak |
| Keywords: | wine, BSA, DNA, black grape, white grape |
| Abstract:
Grapevines in Italy are largely grown for the production of both red and white V.Q.P.R.D. wines. Grape berry external color can be white, red or black due to the presence of anthocyanin pigments in the epicarp cells. Some authors have suggested that color is controlled by two pairs of genes with epistatic action. The use of molecular markers can be an objective method for identification of characters such as color in the selection of new varieties and for the characterization of vegetatively propagated material. It can also be helpful to assist breeders in selecting desirable genotypes. Studies for the identification of DNA markers for berry color were carried out using RAPD analysis and Bulk Segregant Analysis (BSA); this method was chosen for the speed and reliability of the results even on young material. BSA is used to find specific markers for certain characters within pools of individuals or progenies. Twenty winegrape cultivars, 10 with white and 10 with black berries, were examined. DNA was extracted from young leaves harvested in a collection field located in Grugliasco (Piemonte). A total of 120 oligonucleotide primers (10-mers) from Operon Technologies kits A, I, J, M, O, P, and Y were tested on two pools of DNA isolated, respectively, from the white-berried and black-berried cultivars. Twenty-three of the tested primers produced polymorphic bands in the size range between 250 bp and 3000 bp. Preliminary analyses of BSA apparently detected a specific RAPD marker for white color in the kit M. | |
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